JRC GMO-Amplicons | EURL GMFF

Record ID:	MT471102.1
Database:	nt	Go to original record
Description:	Coix lacryma-jobi var. puellarum isolate ZI000287 chloroplast, complete genome.
Taxon:	Coix lacryma-jobi var. puellarum
Original size:	140863 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49222	49745	+	524 bp

Amplicon:

524 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaacaagtg gttctcaaac
151   tagaacccaa aggaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacgaatcga agtaataacg attaatcaca gaacccatat tataatatag
251   gttctttatt ttatttttag aatgaaatta ggaatgatta tgaaatagaa
301   aattcataat ttttttagaa ttattgtgaa tctattccaa tcgaatattg
351   agtaatcaaa tccttcaatt catagttttc gagatctttt aaaaagtgga
401   ttaatcggac gaggataaag agagagtccc attctacatg tcaatactga
451   caacaatgaa atttctagta aaaggaaaat ccgtcgactt tataagtcgt
501   gagggttcaa gtccctctat cccc

View other 6 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.36e-08
primerB	100.0	20 bp	0	0	524	505	2.36e-08

Match details