JRC GMO-Amplicons | EURL GMFF

Record ID:	MW238408.1
Database:	nt	Go to original record
Description:	Mazus pumilus chloroplast, complete genome.
Taxon:	Mazus pumilus
Original size:	152855 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46686	47251	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaaa
101   agtgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aaaagaaaag gataggtgca gagactcaat ggaagctatt
201   ctaacaaatg gagttgactg cattggtaga ggaatctttc catcaaaact
251   gcagaaagga tgaaggataa acgtatctat tgaatactat atcaattaat
301   gatggcccaa atctgtatct gtatttttta atctgaaaag tggaagaatt
351   ggtgtgaatt gattccacat tgaagaaaga atcgaatatt aattcatcaa
401   atcattcact ccatagtccg acagatcttt taaagaactt attaaatcgg
451   gcgagaataa agatagagtc ccattctaca tgtcaatatt ggcaacaatg
501   aaatttatag taagaggaaa atccgtcgac ttttaaaatc gtgagggttc
551   aagtccctct atcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details