JRC GMO-Amplicons | EURL GMFF

Record ID:	MW167297.1
Database:	nt	Go to original record
Description:	Bougainvillea spectabilis chloroplast, complete genome.
Taxon:	Bougainvillea spectabilis
Original size:	154520 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48306	48920	+	615 bp

Amplicon:

615 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgcaaatttt caaattcaga gaaaccctgg aattaaaaaa
101   atgggcaatc ctgagccaac tccttttttc ttttcaaaag aaaaggttca
151   gaaagcaaga aaaaaaggat aggtgcagag actcaaagga agctgttcta
201   acaaatgggg ttgactgtgc tgtgttgtta tcagaattct tccatcaaaa
251   ttccaatcca aaaaaaaagg gtgaagcata tacgtactca actatatcaa
301   ataattaata acaacctgaa tcggtatttt ttttttctca tttttatata
351   tttatgaaat gaaaaaattt atatgagaaa aaaatcgaag aattcttgtg
401   attgtgaatc gattccaagt tgaaaaaaga atcaaatatt cattcatcaa
451   accattcact ccatagtctg atagattttt tgaagaactg attaatcgga
501   cgagaatgaa gagagagtcc agttctacat gtcaatactg acaacaatga
551   aatttataat aagaagaaaa tccgtcgact ttaaaaatcg tgagggttca
601   agtccctcta tcccc

View other 4 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	615	596	2.77e-08

Match details