JRC GMO-Amplicons | EURL GMFF

Record ID:	MT012421.1
Database:	nt	Go to original record
Description:	Salvia yangii chloroplast, complete genome.
Taxon:	Salvia yangii
Original size:	151473 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46460	47023	+	564 bp

Amplicon:

564 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa aaggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgcgccggt agaggaatct ttccatggaa actttagaaa
251   ggatgaagga taaacgcatc tattgaatac tatatcaaat gattaatgtt
301   gacccgaatc tgttttttaa taggaaaatg aaaaaatgga aaaataggtg
351   ggaatttatt tcacgttgaa gaataaatcg aatattcatc aactcattca
401   ctccatagtc cgatagatct tttaaagaac ttattaatcg gacgagaata
451   aagatagagt cccgttctac atgctacatg tcaataccgg caacaatgaa
501   atttatagta agaggaaaat ccgtcgactt taaaaatcgt gagggttcaa
551   gtccctctat cccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	564	545	2.54e-08

Match details