JRC GMO-Amplicons | EURL GMFF

Record ID:	MW149076.1
Database:	nt	Go to original record
Description:	Callicarpa longifolia var. floccosa voucher CY Wang PS 20191002 chloroplast, complete genome.
Taxon:	Callicarpa longifolia var. floccosa
Original size:	154285 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48051	48612	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa aaggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgcgttggt agaggaatct ttccatggaa acttcagaaa
251   ggatgaagga taaacgtatc tattgaatac tatatcaaat gattaatgat
301   ggcccgaatc tgtatctgta ttttttaata tgaaaaatgg aagaattggt
351   gtgaattgat tccacattga agaaaaaatc gaatattcat tcatcaaatc
401   attcactcca taggccgata gatcttttaa agaactgatt aatcggacga
451   gaataaagat agagtcccat tctacatgtc aataccggca acaatgaaat
501   ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt
551   ccctctatcc cc

View other 6 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details