JRC GMO-Amplicons | EURL GMFF

Record ID:	MW075109.1
Database:	nt	Go to original record
Description:	Phyllostachys heteroclada f. solida chloroplast, complete genome.
Taxon:	Phyllostachys heteroclada f. solida
Original size:	139678 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49195	49808	+	614 bp

Amplicon:

614 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagt ggttctcgaa
151   ctagaatcca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg agttgattac gttgtgttgg tagtggaact ctctctaaat
251   ttagagaaag taagggcttt atacatctaa tacacacgta tagatactga
301   catagcaaac gattaatcac ggaacccata tcataatata ggttctttat
351   tcttttttag aatgaaatta ggaatgatta tgaaatagaa aattctgaat
401   ttttttagaa ttattgtgaa cccattccaa tcgaatattg agtaatcaaa
451   tccttcaatt catagttttc gagatctttt aaaaagtgga ttaatcggac
501   gaggataaag agagagtccc attctacatg tcaatactga caacaatgaa
551   atttctagta aaaggaaaat ccgtcgactt tataagttgt gagggttcaa
601   gtccctctat cccc

View other 44 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	614	595	2.77e-08

Match details