JRC GMO-Amplicons | EURL GMFF

Record ID:	MW007383.1
Database:	nt	Go to original record
Description:	Rehmannia glutinosa isolate jk1 chloroplast, complete genome.
Taxon:	Rehmannia glutinosa
Original size:	153499 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47421	47983	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaacccagg aattaagaaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaata aagggatagg tgcagagact caatggaaga tgttctaaca
201   aatggggttg actgcgttgg tagaggaatc tttccaccga aacttcagaa
251   aggataaagg ataaacgtat ctattgaata ctatatcaaa tgattaatga
301   tggcccgaat ctgtatctgt attttgtaat atgaaaaatg aaagaattgg
351   tgtgaattga ttccacattg aagaaagaat caaatattca ttcatcaaat
401   tattcactcc atagtccgat agatctttta aagaactgat taatcggacg
451   agaataaaga tagagtccca ttctacatgt caatatcgac aacaatgaaa
501   tttctagtaa gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag
551   tccctctatc ccc

View other 11 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details