JRC GMO-Amplicons | EURL GMFF

Record ID:	JF904755.1
Database:	nt	Go to original record
Description:	Alopecurus hitchcockii voucher Peterson 18220 (RSA) trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Alopecurus hitchcockii
Original size:	1757 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	851	1415	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtattttga gaaaacctgg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtattg ttagtggaat tccttctaaa
251   ttcgagaaag aagggcttta tacatgtaat aaacacgtat agatactgac
301   atagcaaacg attaatcaca gaacctatat tataatatat ggttctttat
351   tcttttttag aattattgtg aatacattcc aatcgaatat ttagtaatca
401   aatgcttcaa ttcaaagttt tgaggtcttt aaaaaagtgg attaatcgga
451   cgaggacaaa gagagagtcc cattctacat gtcaatactg acaacaatga
501   aatttctagt aaaaggaaaa tccgtcgact ttataagtcg tgagggttca
551   agtccctcta tcccc

View other 4 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details