JRC GMO-Amplicons | EURL GMFF

Record ID:	AY319187.1
Database:	nt	Go to original record
Description:	Melodorum cf. fruticosum Mols 10 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Melodorum cf. fruticosum Mols 10
Original size:	965 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	568	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tgagaacttc caaattcaga gaaaccctgg aattaaagat
101   gggcaatcct gagccaaatc ctgtgttcag aaaacaaggt tcagaaagcg
151   agaatcaaaa aaggataggt gcagagactc aatggaagct gttctaacaa
201   atagagttga ctgcattggt aagggaattc tttccttcta tccaaacgac
251   agaaaggatg atcttgtata cgtacgtata catattgaaa catcaaacga
301   ttaatcacga cttgaatcag tatttatctc tgaaaaattt cagaatagaa
351   agattgtgaa ttgattccaa gttgaaggaa gaatcgaata ttcagagata
401   aaatcattca ttccctagtc taatagatct tttgaagaac cgattaatcg
451   gacgagaata aagatagagt cccgttctac atgtcaatac agacaacaat
501   gaaattgata gtaagaggaa aatccgtcga ctttataaat cgtgagggtt
551   caagtccctc tatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details