JRC GMO-Amplicons | EURL GMFF

Record ID:	MN488594.1
Database:	nt	Go to original record
Description:	Meconopsis punicea isolate DLSY25 chloroplast, complete genome.
Taxon:	Meconopsis punicea
Original size:	153287 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	105969	106568	-	600 bp

Amplicon:

600 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctattttcag aaaaaattta agggatttct
151   gaaagcgaga ataaaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaatgaatg gagttgactg cgttggtaga ggaatccttc tattaaattt
251   taatttaaac tccataaaga atgagggatg aacctatata cgatacgtat
301   gtatacgtac tgaaatataa aagattctat taatgccacc ctaaatataa
351   aaatttttta cataaaaaaa ggaataattt ttgtggatct attccaagtt
401   gaaggaagaa tagaaaattc agttatcaaa ccattcgctc catagtctga
451   tagatctttt gacgaactga ttaatcggac gagaataaag atagagtccc
501   gttctacatg tcaatatcaa taccgacaac aatgaaattt atagtaagag
551   gaaaatccgt cgactttata aatcgtgagg gttcaagtcc ctctatcccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.70e-08
primerB	100.0	20 bp	0	0	600	581	2.70e-08

Match details