JRC GMO-Amplicons | EURL GMFF

Record ID:	AB235050.1
Database:	nt	Go to original record
Description:	Tetrastigma cf. delavayi 7443 chloroplast DNA, trnL-trnF IGS, isolate: 7443, partial sequence.
Taxon:	Tetrastigma cf. delavayi 7443
Original size:	989 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	553	+	553 bp

Amplicon:

553 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct taatatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccagaa aacgctaata
151   aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa caaaaacgga
201   gttgactatg ttacgatgtt aaacgaatcc ttccatcaaa actccagaac
251   cagaaaggat gaaggataaa cctatataca taggtactga aatattatat
301   caaatgatta atgatggctc gaatctctat ttttttatat gaaaaatgaa
351   agaattgttg taaatcgatt ccaatattca ttgatcaaat cattcactcc
401   atagtccgat agatcatttg aagaacggat taatcggacg agaataaaga
451   gagagtccca ttctacatgt caatatcgac agcaatgaaa tttatagtaa
501   gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag tccctctatc
551   ccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	553	534	2.49e-08

Match details