JRC GMO-Amplicons | EURL GMFF

Record ID:	AB235049.1
Database:	nt	Go to original record
Description:	Rhoicissus rhomboidea chloroplast DNA, trnL-trnF IGS, isolate: H26, partial sequence.
Taxon:	Rhoicissus rhomboidea
Original size:	1037 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	595	+	594 bp

Amplicon:

594 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct taatatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aataaaaaat
101   ggggcaatcc tgagccaaat cctgttttcc gaaaacaacc aagggttcag
151   aaaacgataa taaaaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacaaaaa caaatggagt tgactatgtt gcgttgttaa aggaatcctt
251   ctgtcaaaac tccagaacca gaaaggatga aggataaacc tatatacata
301   ggtactgaaa gattatatca aataattaat gacggctcga atctgtattt
351   tttgatatga aaaatgaaag aattattgta aatcgattcc aagttgaaga
401   aagaaaagaa tcgaatattc attgatcaaa tcattcactc catagtccga
451   tagatctttt gaagaactga ttaatcggac gagaataaag atagagtccc
501   attctacatg tcaataccga caacaatgaa atttatagta agaggaaaat
551   ccgtcgactt taaaaatcgt gagggttcaa gtccctctat cccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	594	575	2.68e-08

Match details