JRC GMO-Amplicons | EURL GMFF

Record ID:	AB235011.1
Database:	nt	Go to original record
Description:	Cissus discolor chloroplast DNA, trnL-trnF IGS, isolate: 7468, partial sequence.
Taxon:	Cissus discolor
Original size:	1025 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	586	+	586 bp

Amplicon:

586 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct taatatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ccgttttccg aaaacgacca agggttcaga
151   aaactataat aaaaaaggat aggtgcagag actcaacgga agctattcta
201   acaaaaacaa atggagttga ctatgttgcg ttgttaaacg aatccttcca
251   tcaaaattcc agaaccagaa aggatgaagg ataaacctat atacataggt
301   actgaaatat tatatcaaat gattaatgat ggctcgagtc tgtatttttt
351   gatatgaaaa atgaaagaat tgttgtaaat agattacaaa gaaaagaatc
401   gaatattcat tgctcaaata attcactcca tagtccgata gataaatctt
451   ttgaagaact gattaatcgg acgagaataa agatagagtc ccattctaca
501   tgtcaatacc gacaagaatg aaatttatag taagaggaaa atccgtcgac
551   tttaaaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	586	567	2.64e-08

Match details