Tested in:	Potato
Genetic target:	trnL
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu (trnL) intron (ISO 21569:2005)
Comments:	Plant-specific methods may generate amplicons with different sequence or length in different plant species or plant varieties.

Specificity:	Plant specific
Analysis type:	Qualitative
Assay:	Endpoint PCR (Simplex)
Amplicon sequence:	CGAAATCGGTAGACGCTACGGACTTAATTGGATTGAGCCTTGGTATGGAAACTTACTAAGTGATCACTTTCAAATTCAGA GAAACCCTGGAATTAACAAAAATGGGCAATCCTGAGCCAAATCCTGTTTTCTGAAAACAAACAAAGGTTCAGAAAAAAAG GATAGGTGCAGAGACTCAATGGAAGCTATTCTAACAAATGGAGTTAAATGCGTTGGTAGAGGACTCTTTACATCGAAACT TCAGAAAGAAAAAGAATGAAGTGAAGGATAAACGTATATACATACGTATTGAATACTATATCAAATGATTAATGACGACC CGAATCCGTAGTTTTTCTATAAAAAATCGAAGAATTGGTGTGAATCCATTCTACATTGAAGAAAGAATCGAATATTCATT GATCAAATCATTCACTCCATAGTCTGATAGATCTTTTGAAGAACTGATTAATCGGACGAGAATAAAGATAGAGTCCCGTT CTACATGTCAATACCGGCAACAATGAAATTTATAGTAAAAGGAAAATCCGTCGACTTTAAAAATCGTGAGGGTTCAAGTC CCTCTATCCCC Genbank ID: DQ131549
Amplicon size:	571 bp
Oligos:

Type	Name(s)	Sequence	Length	Target
Primer forward	A1 /	CGAAATCGGTAGACGCTACG	20 bp	trnL
Primer reverse	A2 /	GGGGATAGAGGGACTTGAAC	20 bp	trnL