JRC GMO-Amplicons | EURL GMFF

Record ID:	AB234983.1
Database:	nt	Go to original record
Description:	Ampelocissus gracilis chloroplast DNA, trnL-trnF IGS, isolate: 7512, partial sequence.
Taxon:	Ampelocissus gracilis
Original size:	1012 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	597	+	597 bp

Amplicon:

597 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagtct taatatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ctgttttccg aaaacaacca agggttcaga
151   aaacgataat aaaaaaagga aaggataggt gcagagactc aatggaagct
201   gttctaacaa aaacaaatgg agttgactat gttgcgttgt taaacgaatc
251   cttccatcaa aactccagaa ccagaaagga tgaaggataa acctatatac
301   ataggtactg aaatattata tcaaatgatt aatgatggct cgaatctgta
351   ttttttgata tgaaaaatga aagaattgtt gtaaatcgat tccaagttga
401   agaaagaaaa gaatcgaata ttcattgatc aaatcattca ctccatagtc
451   cgatagatct tttgaagaac tgattaatcg gacgagaata aagatagagt
501   cccattctac atgtcaatac cgacaacaat gaaatttata gtaagaggaa
551   aatccgtcga ctttaaaaat cgtgagggtt caagtccctc tatcccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	597	578	2.69e-08

Match details