JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ620153.1
Database:	nt	Go to original record
Description:	Peponidium horridum chloroplast trnT-trnL IGS (partial), tRNA-Leu (UAA) gene and trnL-trnF IGS (partial), specimen voucher Labat et al. 2236 (UPS)
Taxon:	Peponidium horridum
Original size:	1729 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	801	1352	+	552 bp

Amplicon:

552 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaag ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttggct gcgttagtag agaaatcttt ccatcgaaaa ttccgaaagg
251   ataaaacgta tacgtattga atactatatc aaatgattaa tgacgactcg
301   actgaatctg ttttttttat atgaaaatgg aagaattggt gtgaatagat
351   tccacattga agaaagaatc gaatattcat tgattaaatg attcactcca
401   tagtctgata gatcttttca agaattgatt aatcggacga gaataaagat
451   agagtcccgt tctacatgtc aatgtcggca acaatgaaat ttatagtaat
501   aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt ccctctatcc
551   cc

View other 6 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	552	533	2.48e-08

Match details