JRC GMO-Amplicons | EURL GMFF

Record ID:	AF430736.1
Database:	nt	Go to original record
Description:	Caesalpinia sclerocarpa chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Libidibia sclerocarpa
Original size:	1058 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	597	+	597 bp

Amplicon:

597 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagggaaagg gagaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcgtttcg ttagtaaagg aatccttcca
251   tcgaaactcc agaaaagaaa ggatcaagga tgaacatata tatacgtact
301   gaaatactat ttcaattgat tagaccagac agaccagacc ccaaatctct
351   attttttaat atttctatga caaatgaaag atgtgaatag attccaagtt
401   gaagaaagaa tcgaatattt attgataaaa tcattcactc catcatagtc
451   tgatagatct tttgaagaac tgattaatcg gacgagaata aagatagagt
501   cccattctac atgtcaatac cgacaacaat gaaatttata gtaagaggaa
551   aatccgtcga ctttagaaat cgtgagggtt caagtccctc tatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	597	578	2.69e-08

Match details