JRC GMO-Amplicons | EURL GMFF

Record ID:	JF922143.1
Database:	nt	Go to original record
Description:	Cedrela fissilis isolate RSSMA73 trnT-trnL intergenic spacer and tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Cedrela fissilis
Original size:	1498 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	904	1498	+	595 bp

Amplicon:

595 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aatcaaaaat
101   gggcaatcct gagccaaatc ctgttttaca agaacaaaca agggttcaga
151   aagcgaaaaa aaagggggat aggtgcagag actcaatgga agctgttcta
201   agaaatgggg ttgactgcct tttttggtaa agaaaggaaa agaaatcctt
251   ctatcgaata tcgaaactcc ataaaggata agcgtgtata cactatgtat
301   acacaatgaa aaactatctc aaaaatgaca accgaatccg tatttttttt
351   tatgaaaaag aaaagaattg ttatgaatca attccaagtt gaagaaagaa
401   tcgaatattc attgatcaaa tcattcactc cacagtctga tcgatctttt
451   cttttgaaga actgattaat cggacaagaa taaagataga gtcccattct
501   acatgtcaat atcaataccg gcaacaatga aatttagagt aagaggaaaa
551   tccgtcgact ttagaaatcg tgagggttca agtccctcta tcccc

View other 7 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	595	576	2.68e-08

Match details