JRC GMO-Amplicons | EURL GMFF

Record ID:	HM536235.1
Database:	nt	Go to original record
Description:	Glionnetia sericea isolate cA099 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer and tRNA-Leu (trnL-UAA) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Glionnetia sericea
Original size:	1713 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	800	1357	+	558 bp

Amplicon:

558 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaag ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggaattgact gcgttagtag agaaatcttt ccatcgaaaa ttccgaaagg
251   ataaaaaacg tatatatacg tattgaatac tatatcaaat gattaatgac
301   gactcgactg aatctgtatt atttatatga aaacggaaga attggtgtga
351   atagattcca cattgaagaa agaatcgaat attcattgat taaatgattc
401   actccatagt ctgatagatc ttttcaagaa ttgattaatc ggacgagaat
451   aaagatagag tcccgttcta catgccaatg tcggcaacaa tgaaatttat
501   agtaagagga aaatccgtcg actttaaaaa tcgtgagggt tcaagtccct
551   ctatcccc

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	558	539	2.51e-08

Match details