JRC GMO-Amplicons | EURL GMFF

Record ID:	HM536231.1
Database:	nt	Go to original record
Description:	Jackiopsis ornata isolate cA047 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL-UAA) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Jackiopsis ornata
Original size:	1604 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	710	1274	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ccgaaaccaa gggttcagaa
151   agtgaaaaaa ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggct gcgttagtag agaaatcttt ccatcgaaaa ttccgaaagg
251   ataaagtgaa agataaacgt atatacgtat tgaatactat atcaaatgat
301   taatgacgac tcgactgaat ctgtattttt tatatgaaaa tggaagaatt
351   ggtgtgaata gattccacat tgaagaaaga atcgaatatt cattgattaa
401   acgattcact ccatagtctg atagatcttt tcaagaattg attaatcgga
451   cgagaataaa gatagagtcc cgttctacgt gtcaatgtcg gcaacaatga
501   aatttatagt aagaggaaaa tccgtcgact ttacaaatcg tgagggttca
551   agtccctcta tcccc

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details