JRC GMO-Amplicons | EURL GMFF

Record ID:	GQ381985.1
Database:	nt	Go to original record
Description:	Babiana lineolata tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Babiana lineolata
Original size:	775 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	550	+	549 bp

Amplicon:

549 bp

1     cgaaatcggt agacgctacg gacttgattg aattgagcct tagtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aaaaaaaagg
101   gggcaatcct gagccaaatc tttattttga gaaaacaaaa actagaataa
151   aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa cgaatggagt
201   tgactacgtt gtgttggtag ccggaatctt tctatcgaaa tgaaagataa
251   ggatatacct aattcgtata catatttacc atatcaaacg attaatcatg
301   acccaaatcc attatatata tatgcaaaat ggggggagat ctattccaat
351   cgagaagaat cgaatattcg gtgatcaaat ctattctata cattccaggg
401   tttgatagat cttttgaaaa acagattaat cggaagagaa taaagagaga
451   gtcccattat acatgtcaat aacgacaaca atgaaattta tagtaaaagg
501   aaaatccgtc gactttagaa atcgtgaggg ttcaagtccc tctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	549	530	2.47e-08

Match details