JRC GMO-Amplicons | EURL GMFF

Record ID:	AY879119.1
Database:	nt	Go to original record
Description:	Andryala integrifolia voucher Schuhwerk 00/11 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Andryala integrifolia
Original size:	943 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	521	+	521 bp

Amplicon:

521 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgatcacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcacgtttt ccgaaaacaa aaaaaggttc
151   agaaagcgaa aataaaaaag gataggtgca gagactcgat ggaagctgtt
201   ctaacgaatg gagttgattg tcttacgtta ttagagtatt agaggaatcc
251   ttctatcgaa actattgaaa cttcagaagg ataaacctat atacataata
301   cagaagaatt gttgtcaatc gattccatat tgaagaaaga atcgatcatt
351   gatcaaacca ttcactccat aatctgatag atcttttgaa gaactgatta
401   atcggacgag aataaagata gagtcccgtt ctacatgtca ataccggcaa
451   caatgaaatt tatagtaaga ggaaaatccg tcgatttcaa aaatcgtgag
501   ggttcaagtc cctctatccc c

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.34e-08
primerB	100.0	20 bp	0	0	521	502	2.34e-08

Match details