JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ492303.1
Database:	nt	Go to original record
Description:	Saintpaulia velutina chloroplast tRNA-Leu gene (partial), trnL-F intergenic spacer and tRNA-Phe gene (partial)
Taxon:	Streptocarpus ionanthus subsp. velutinus
Original size:	939 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	541	+	541 bp

Amplicon:

541 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgaagacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atccttgttt tctcaaaaca agggttcaga
151   gagcgaaaaa gaggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgcgttggt agaggaattg aaacttcaga aaggatgaaa
251   cgtatctatt gaatactatt gattgatcag gcccacatct gtatctgggt
301   ttttgtatat gaaaaatgga agaattggtg tgaattgatt tcacattgat
351   aaaagaatcg aatattcatt gatcaaatca ttgaccccat agtccgatag
401   gtctcttaaa gaattgatta ttcggacgag aataaagata gagtcccgtt
451   ctacatgtca ataccgacaa caatgaaact tatagtaaga ggaaaatccg
501   tcgactttaa aaatcgtgag ggttcaagtc cctctatccc c

View other 6 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.43e-08
primerB	100.0	20 bp	0	0	541	522	2.43e-08

Match details