JRC GMO-Amplicons | EURL GMFF

Record ID:	EF440556.1
Database:	nt	Go to original record
Description:	Picea omorika tRNA-Thr (trnT) gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Picea omorika
Original size:	1434 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	446	1007	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttgatcg aattgagcct tggtatggaa
51    acctaccaag tgatagcttc caaatccagg gaaccctggg atattttgaa
101   tgggtaatcc tgagccaaat ccggttcatg gagacaatag tttcttcttt
151   tattctccta agataggaag gggataggtg cagagactca atggaagcta
201   ttctaacgaa tgaatctcat ttggtccaat actgtattta tagaacgctc
251   tatttacacc taaaaagtgg gaatgtgata taacatcaga caaaactcgc
301   gatcagaact tgaatcgttc caagcatcta ttcgtaagat agatgccaga
351   ttcgagttga agtactgatt ttacattaag taatccaatt atgaacttct
401   ctactttaga tagagaattg aatcagtttt tggaataaat ggttggacga
451   gaataaagat agagtccaat tctacgtgtc aatgtcaaca acaatgcaaa
501   ttgcagtagg aggaaaatcc gttggcttta tagaccgtga gggttcaagt
551   ccctctatcc cc

View other 13 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details