JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ430874.1
Database:	nt	Go to original record
Description:	Diospyros kaki chloroplast tRNA-Leu gene for transfer RNA-Leu and partial tRNA-Phe gene for transfer RNA-Phe
Taxon:	Diospyros kaki
Original size:	1362 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	384	968	+	585 bp

Amplicon:

585 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgattt tcgaaaacaa acaaagattc
151   agaaagcgaa aataaaaaaa ggataggtgc agagactcaa tggaagctgt
201   tctaacaaat ggagttgact gcgttggtag aggaatcctt ccatcgaaat
251   tttcgaaagg atgaaagaga aacctatata catacgcata cgtactgaaa
301   tactatagca aacgattaat gacgacgcaa atctgtatgc gtattttttt
351   tatatgaaaa tggaagaatt gttgcgaatc gattcgacat tgaataaaga
401   attgactatt cattgatcaa atcatttact ccatagtctg atagatcttt
451   tgaagaactg attaatcgga cgagaataaa gatagagtcc cattctacat
501   gtcaataccg acaacaatga aatttatagt aagaggaaaa tccgtcgact
551   ttaaaaatcg tgagggttca agtccctcta tcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	585	566	2.63e-08

Match details