JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ801113.1
Database:	nt	Go to original record
Description:	Bauhinia forficata voucher Sinou s.n. (MT) tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; plastid.
Taxon:	Bauhinia forficata
Original size:	1043 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	597	+	597 bp

Amplicon:

597 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc cagttttccg aaaacaaaga agagttcaaa
151   aagttagaat aaaaaggata ggtgcagaga ctcaacggaa gctgttctaa
201   caaatggagt tgacgggatt tcgtttcatt agtaaagtaa tccttccatc
251   gaaactccag aaaagaaagg atcaaagata aacgtatata tagtatacgt
301   cccgaaatac tatctcaact aaattaatga cgacgccgaa tctctatttt
351   tgaatattta tataagaaat gaaagatgta aatcgattca aatcaaagtt
401   gaggaaagag tcgaatattc attcatcaaa tcattcactc catcataatc
451   cgatagatct tttgaagaac cgattaatcg gacaagaata aagatagagt
501   cccattctac atgtcaatgc tgacaacaat gaaatttata gtaagaggaa
551   aatccgtcga ctttaaaaat cgtgagggtt caagtccctc tatcccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	597	578	2.69e-08

Match details