JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ801059.1
Database:	nt	Go to original record
Description:	Lysiphyllum hookeri voucher Weston 2445 (NSW) tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; plastid.
Taxon:	Lysiphyllum hookeri
Original size:	991 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	598	+	598 bp

Amplicon:

598 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaac
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaaa agagttcaaa
151   aagtgagaat aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaatggagt tgacgggatt tcgtttcgtt agtaaagtag taatccttcc
251   atcgaaactc cagaaaagaa aggatcgaag ataaacgtat atatacatat
301   acgtaccgaa atactatctc aactaaatga atgacgacgc cgaatctcta
351   ttcattaata tttataatga aagatgatgt aaatggattc aaagttgaag
401   aaaaagtcga atattcattc atcaaatcat tcactccatc ataacataat
451   ccgatatttc ttttgaagaa ctgattaatc ggacaagaat aaagatagag
501   tcccattcta catgtcaata ctgacaacaa tgaaatttat agtaatagga
551   aaatccgtcg actttaaaaa tcgtgagggt tcaagtccct ctatcccc

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	598	579	2.69e-08

Match details