JRC GMO-Amplicons | EURL GMFF

Record ID:	EU792457.1
Database:	nt	Go to original record
Description:	Poa kerguelensis voucher Hennion Gen5 CAN tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Poa kerguelensis
Original size:	1866 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	859	1475	+	617 bp

Amplicon:

617 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ttagtggaat tccttctaaa
251   tttcgaaaaa gaagggcttt atacatctaa taaacacgta tcgatactga
301   catagcaaac gattaatcac agaaccaata ttataatatt ggttctttat
351   tcttttttag aatgaaatag aaatgattat gaaataaaaa attctgaatt
401   tttttagaat tattgtattg tgaatccatt ccaatcgaat atttagtaat
451   caaatccttc aattcaaagt tttgaggtct ttaaaaaagt ggattaatcg
501   gacgaggaca aagagagagt cccattctac atgtcaatac tgacaacaat
551   gaaatttcta gtaaaaggaa aatccgtcga ctttataagt cgtgagggtt
601   caagtccctc tatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	617	598	2.78e-08

Match details