JRC GMO-Amplicons | EURL GMFF

Record ID:	EU817474.1
Database:	nt	Go to original record
Description:	Alberta sambiranensis trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Razafimandimbisonia sambiranensis
Original size:	1671 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	769	1327	+	559 bp

Amplicon:

559 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggttcataa
151   agtgaaaaaa ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggct gcgttagtag agaaatcttt ccatctaaaa ttccgaaagg
251   ataaagtaac gtatatatac gtattgaata ctatatcaaa tgattaatga
301   ggactcgact gaatctgtat tttttatatg aaaacggaag aattggtgtg
351   aatagattcc acattgaaga aagaatcgaa tattcattga tcaaatgatt
401   cactccatag tctgatagat cttttcaaga attgattaat cggacgagaa
451   taaagataga gtcccattct acatgtcaat gtcggcaaca attaaattta
501   tagtaaaagg aaaatccgtc gactttaaaa atcgtgaggg ttcaagtccc
551   tctatcccc

View other 8 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	559	540	2.52e-08

Match details