JRC GMO-Amplicons | EURL GMFF

Record ID:	EU817454.1
Database:	nt	Go to original record
Description:	Augusta austrocaledonica trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Augusta austrocaledonica
Original size:	1703 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	777	1343	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acgcactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaacaa aggttcagaa
151   agttaaaaaa ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggct gcgttagtag ataaatcttt ccatctaaaa ttccgaaagg
251   ataaagtgaa ggatacacgt atatattacg tattgaatac tatatcaaat
301   gattaatgac gactcgaatg aatctgtatt tttttatgaa aacggaagaa
351   ttggtgtgaa tagattccac attgaagaaa gaatcgaata ttaattgatc
401   aaatgattca ctccatagtc tgatacatct tttcaagaat tgattaatcg
451   gacgagaata aagatagagt cccgttctac atgtcaatgt cggcaacaat
501   gaaatttata gtaagaggaa aatccgtcga ctttaaaaat cgtgagggtt
551   caagtccctc tatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details