JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ467933.1
Database:	nt	Go to original record
Description:	Carica papaya transgenic cultivar Rainbow beta-glucuronidase (uidA), coat protein, and neophosphotransferase genes, complete cds.
Taxon:	Carica papaya
Original size:	15595 bp

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Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	13987	14578	+	592 bp

Amplicon:

592 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttacg agaacaaaca aaacaagagt
151   tcagaaagcg agaaaagggg gataggtgca gagactcaat ggaagctgtt
201   ctaacaaatg gagttcatta ccttgtgttg gtaaaggaat ccttctatcg
251   aacctccaga aaagatgaag ggataacctt atctaaatag gtataagtaa
301   tttaaaacta tctcaaaaat ggtgacccga atatggattt tttttttttt
351   ttatatgaaa aaaaaagaat tgttttgttg tgaatcaatt caaagttgaa
401   gaaaaaatcg aatattcatt gatcaaacca ttcactccat agtctgatag
451   atcttttcaa gaactgatta atcggacgag aataaagata gagtcccatt
501   ctacatgtca atagcgacaa caatgaaatt tatagtaaga tgaaaatccg
551   tcgacttaaa aaagtcgtga gggttcaagt ccctctatcc cc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	592	573	2.67e-08

Match details