JRC GMO-Amplicons | EURL GMFF

Record ID:	EU556530.1
Database:	nt	Go to original record
Description:	Thymus mandschuricus isolate HM tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Thymus mandschuricus
Original size:	898 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	53	595	+	543 bp

Amplicon:

543 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tagtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggataggtg
151   cagagactca atggaagctg ttctaacaaa tggagttgac tgcgttggta
201   gaggaatctt tccatggaaa ctttataaag gatgaaagat aaacgcatct
251   attgaatact atatcaaatg attaatgttg gcccgaatct atttttttat
301   atgaaaatga aaaaatggaa aaatcggtgt gaatttattt cacgttgaag
351   aaaaaataga atattcatca actcattaac tccataatcc gatagatctt
401   ttaaagaact ttttaatcgg gcgagaataa agatagagtc ccattctaca
451   tgctacatat caataccggc aacaatgaaa tttatagtaa gaggaaaatc
501   cgtcgacttt aaaaatcgtg agggttcaag tccctctatc ccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.44e-08
primerB	100.0	20 bp	0	0	543	524	2.44e-08

Match details