JRC GMO-Amplicons | EURL GMFF

Record ID:	AM909708.1
Database:	nt	Go to original record
Description:	Ornithogalum arabicum chloroplast tRNA-Leu gene (partial), trnL-trnF IGS and tRNA-Phe gene (partial), specimen voucher MP-H597
Taxon:	Ornithogalum arabicum
Original size:	1053 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	13	610	+	598 bp

Amplicon:

598 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tagtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aactaaaaat
101   gggcaatcct gagccaaatc tttatgttta gaaaaacaag ggtttaaaaa
151   ctagactaga ataaaaaggg ataggtgcag agactcaatg gaagctgttc
201   taacgaatgg agttgactac gttgcattga taataggaat ccttctatta
251   tcgaaattaa aaaaaggtga cccatatatc taatacgtac gtatacatac
301   tgacatatca aacgattaat cacgactcga atccatatac atatattata
351   tatatgcgca atatatgcaa aattaagtgt ggatctattc caatcgaagt
401   tgacggaaga atcaaatatt cagtgatcaa atcattcatt ccagagttta
451   atagaccctt ttttgaaaaa ctgattaatc ggacgagaat aaagagagag
501   tcccattcta catgtcaata ccgacaacaa tgaaatttat agtaagagga
551   aaatccgtcg actttagaaa tcgtgagggt tcaagtccct ctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	598	579	2.69e-08

Match details