JRC GMO-Amplicons | EURL GMFF

Record ID:	EU828208.1
Database:	nt	Go to original record
Description:	Aureolaria pedicularia isolate 3 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Aureolaria pedicularia
Original size:	1571 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	663	1208	+	546 bp

Amplicon:

546 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaagaaa
101   aaagggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgat tgcgctgata gaggaatgaa acttcagaaa ggataaatgt
251   atctattgaa tactatatca aaatcaaatg attgattaat gatggcccaa
301   atctagatct gtatttttga atacgaaaag tggaagaatt ggtgtgaatt
351   tcttccataa agaatattca ttcatcaaat cattcactcc atagtccgat
401   agatcttttt taaaagatct gattaatcgg acgagaataa agatagagtc
451   ccattctaca tgtcaatacc ggcaacaatg aaatttatag taaaaggaaa
501   atccgtcgac ttttaaaatc gtgagggttc aagtccctct atcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.46e-08
primerB	100.0	20 bp	0	0	546	527	2.46e-08

Match details