JRC GMO-Amplicons | EURL GMFF

Record ID:	EU828171.1
Database:	nt	Go to original record
Description:	Agalinis obtusifolia isolate 13AL tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Agalinis obtusifolia
Original size:	1654 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	726	1283	+	558 bp

Amplicon:

558 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaagaaa
101   aaagggcaat cctgagccaa atcctctttt cttaaaacaa aagttcagaa
151   aacgaaaaaa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgat tgcgctgata gaggaatgaa acttcagaaa ggatgaagga
251   taaacgtatc tattgaatac tatatcaaaa tcaaatgatt gattaatgat
301   ggcccaaatc tctatctgta tttttgaata cgaaaagtgg aagaattggt
351   gtgaatttct tccctaaaga atattcattc atcaaatcat tcactccata
401   gtccggtccg atagatcttt tttaaaagat ctgattaatc ggacgagaat
451   aaagatagag tcccattcta catgtcaata ccggcaacaa tgaaatttat
501   agtaaaagga aaatccgtcg acttttaaaa tcgtgagggt tcaagtccct
551   ctatcccc

View other 7 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	558	539	2.51e-08

Match details