JRC GMO-Amplicons | EURL GMFF

Record ID:	EU828168.1
Database:	nt	Go to original record
Description:	Agalinis maritima isolate 2MA tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Agalinis maritima
Original size:	1662 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	725	1277	+	553 bp

Amplicon:

553 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaagaaa
101   taaagggcaa tcctgagcca aatcctgttt tcttaaaaca aaagttcaga
151   aaacgaaaaa aaggataggt gcagagactc aatggaagct gttctaacaa
201   acggagttga ttgcgctgat agaggaatga aacttccgaa aggatgaagg
251   ataaacgtat ctatggaata gatatcaaaa tcaaatgatt gattaatgat
301   ggcccaaatc tctatctgta tttttgaata cgaaaagtgg aagaattggt
351   gtgaatttct tccataaaga atattccttc atcaaatcat tcactccgta
401   gtccgataga tctttttaaa aagatctgat taatcggacg agaataaaga
451   tagagtccca ttctacatgt caataccggc aacaatgaaa tttataggaa
501   aaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag tccctctatc
551   ccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	553	534	2.49e-08

Match details