JRC GMO-Amplicons | EURL GMFF

Record ID:	EU828153.1
Database:	nt	Go to original record
Description:	Agalinis filicaulis isolate 1AL tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Agalinis filicaulis
Original size:	1660 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	746	1296	+	551 bp

Amplicon:

551 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaagaaa
101   aaagggcaat cctgagccaa atcctgtttt cttaaaacaa aagttcagaa
151   aacgaaacga aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaatggagt tggttgcgat gatagaggaa tgaaacttca gaaaggatga
251   aggataaacg tatctattga atactctatc aaaatcatga ttaatgatgg
301   cccaaatctc tatctgtatt tttgaatacg aaaagtggaa gaattggtgt
351   gaatttcttc cctaaagaat attcattcat caaatcattc actccatagt
401   ccgatagatc ttttttaaaa gatctgatta atcggacgag aataaagata
451   gagtcccatt ctacatgtca atatgggcaa caatgaaatt tttagtaaaa
501   ggaaaatccg tcgacttttc aaatcgtgag ggttcaagtc cctctatccc
551   c

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	551	532	2.48e-08

Match details