Match details

Record ID: EU828130.1
Database: nt Go to original record
Description: Agalinis acuta isolate 13PCMA tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon: Agalinis acuta
Original size: 1672 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 732 1283 + 552 bp

Amplicon: 552 bp
1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaagaaa
101   aaagggcaat cctgagccaa atcctgtttt cttaaaccaa aagttcagaa
151   aacgaaaaaa ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttgatt gcgctgatag aggaatgaaa cttcagaaag gatgaaggat
251   aaacgtatct attgaatact atatcaaaat caaatgattg attaatgatg
301   gcccaaatct ctatctgtat ttttgaatac gaaaagtgga agaattggtg
351   tgaatttctt ccataacgaa tattcattca tcaaatcatt cactccatcg
401   tccgatagat cttttttaaa agatctgatt aatcggacga gaataaagat
451   agagtcccat tctacatgtc aataccggca acaatgaaat ttatagtaaa
501   aggaaaatcc gtcgactttt aaaatcgtga gggttcaagt ccctctatcc
551   cc 

View other 21 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.48e-08
primerB 100.0 20 bp 0 0 552 533 2.48e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.