JRC GMO-Amplicons | EURL GMFF

Record ID:	EF571562.1
Database:	nt	Go to original record
Description:	Junellia uniflora voucher R. G. Olmstead 2004-155 (WTU) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Junellia uniflora
Original size:	1593 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	672	1224	+	553 bp

Amplicon:

553 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgacaaag ggggataggt gcagagactc aatggaagtt gttctaacaa
201   atggagttga ctgtgtgggt agaggaatct ttccatcgaa acttccgaaa
251   ggatgaagga taaacgtatc tattgaatac tctatcaaat gattaatgag
301   gtgtatctgt attttttgat atgaaaaatg gaagaattgg tgtgaattga
351   ttccacattg aagaaagaat cgaatattcc ttgatcaact cattcactcc
401   atagtccgat aaatctttta aagaactgat taatcggacg agaataaaga
451   tagagtccca ttctacatgt caatatcggc aacaatgaaa tttattgtaa
501   gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag tccctctatc
551   ccc

View other 4 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	553	534	2.49e-08

Match details