JRC GMO-Amplicons | EURL GMFF

Record ID:	MN202613.1
Database:	nt	Go to original record
Description:	Calligonum korlaense voucher XJBI:50852 chloroplast, complete genome.
Taxon:	Calligonum korlaense
Original size:	161316 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49701	50294	+	594 bp

Amplicon:

594 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aattaaaaaa
101   aatgggcaat cctgagccaa ctccttcttt ccaaaaggaa gaataaaaaa
151   gggataggtg cagagactca atggaagctg ttctaacaaa tggagttaac
201   ggtcttgcgt tgttataaga attcttccat cgaaactcca aaaaggatga
251   agaataaacc tatacgcata catacgtaaa tataaatact acatctatat
301   taatgaaatt caaaaagaaa atcttgatga cgacccgaat ttttgtttta
351   tgactatgaa aaaatggaag aattgttgtg aatcgattcc aagttgagga
401   aagaatcgaa tattcgttta gtaaattaaa acatctagtc cccagtctga
451   tagatctttt gaagaaccga tccatcggat gagaatgaag atagagtccc
501   attctacgtg tcaatcctga caacaatgaa atttatagta agaggaaaat
551   ccgtcgactt tataaatcgt gagggttcaa gtccctctat cccc

View other 9 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	594	575	2.68e-08

Match details