JRC GMO-Amplicons | EURL GMFF

Record ID:	MN480777.1
Database:	nt	Go to original record
Description:	Puccinellia tenuiflora voucher Alt285 tRNA-Leu gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Puccinellia tenuiflora
Original size:	906 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	611	+	611 bp

Amplicon:

611 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacctgg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ttagtggaat tccttctaaa
251   ttcgagaagg aagggcttta tacatctaat aaacacctat agatactgcc
301   atagcaaagg attaatcaca gaacccatat tataatatag gttctttatt
351   cttttttaga atcaaataga aatgattatg aaataaaaaa ttcataattt
401   ttttagaatt attgtgaatc cattcccatc gaatatttag taatcaaatc
451   cttcaattca aagttttgag gtctttaaaa aagtagatta atcgaacgag
501   gacaaagaga gagtcccatt ctacatgtca atactgacaa caatgaaatt
551   tctagtaaaa ggaaaatccg tcgactttat aagtcgtgag ggttcaagtc
601   cctctatccc c

View other 7 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	611	592	2.75e-08

Match details