JRC GMO-Amplicons | EURL GMFF

Record ID:	MN814851.1
Database:	nt	Go to original record
Description:	Ajuga campylantha chloroplast, complete genome.
Taxon:	Ajuga campylantha
Original size:	150478 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	115526	116081	-	556 bp

Amplicon:

556 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctgctaag tgataacttt caaattcaga gaaacccagg aactaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa agggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttca ctgcgctggt agaggaatct ttccatggaa acttcagaaa
251   ggatgaagga taaatggatc tattgaatac tatatcgaat gattcacgat
301   ggcccgaatc tgtatttttt aatatgaaaa atggaagaat tggtgtgaat
351   tgattccaca ttgaagaaaa aatcgaatat tcattcatca aatcattcac
401   tccatagtcc gatagatctt ttcaagaact gattaatcgg acgagaataa
451   agatagagtc ccgttctaca tgtcaatacc ggcaacaatg aaatttatag
501   taataggaaa atccgtcgac tttaaaaatc gtgagggttc aagtccctct
551   atcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	556	537	2.50e-08

Match details