JRC GMO-Amplicons | EURL GMFF

Record ID:	MH593367.1
Database:	nt	Go to original record
Description:	Fritillaria usuriensis isolate GZDQ26-1 chloroplast, complete genome.
Taxon:	Fritillaria usuriensis
Original size:	151571 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45116	45720	+	605 bp

Amplicon:

605 bp

1     cgaaatcggt agacgctacg gacttgatta aattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttattttga gaaaaagggt ttaatttata
151   gtttttataa tataaaacta caataaaaaa agataggtgc agagactcaa
201   tggaagctgt tctaacgaat gaagttgatt acgttgcgct ggtagccgga
251   atccttctat caaaattacg gagaggatgc ccgccctata tacgtatata
301   tacatattga catagtaaac gattgattaa tcgcggcccg aatccattat
351   aatatatata tgaaaaatat aagagttatt gtaaatctat tccatattcc
401   aatcaaagtt taaggaagaa tcgaatattc agtgatcaaa ttattcattc
451   cagagtctgt atagatcttt ttaaaaactg attattcgga cgagaataaa
501   gagagagtcc cattctacgt gtcaataccg acaacaatga aatttatagt
551   aaaaggaaaa tccgtcgact ttataagtcg tgagggttca agtccctcta
601   tcccc

View other 7 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	605	586	2.73e-08

Match details