JRC GMO-Amplicons | EURL GMFF

Record ID:	MT211647.1
Database:	nt	Go to original record
Description:	Trichosanthes kirilowii voucher YSG_KIOM-2019-66 chloroplast, complete genome.
Taxon:	Trichosanthes kirilowii
Original size:	156791 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49805	50389	+	585 bp

Amplicon:

585 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaaaaaaagg gtaggtgcag
151   agactcaatg gaagctgttc taacaaatgg agttgactac gttgcgttag
201   taaaggaatg aatccttcta tcgaaactcc agaaaagatg aaagataaac
251   gtattacgta ctgaaatact atttgattaa tgacaacccg aatctccatt
301   tttttttttt ttatttatat aaaaaatgat atataaaaat gaacgaattg
351   ttctgaatcg attccaatat ctccaagttg aaaaaagaat cgaatattca
401   ttgatcaaat catttactcc atcatacata gtctgataga tcttttgaag
451   aactgattaa tcagatgcga atagaataaa gatagagtcc cattctacat
501   gtcaataccg acaaaaatga aatttatagt aagaggaaaa tccgtcgact
551   ttaaaaatcg tgagggttca agtccctcta tcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	585	566	2.63e-08

Match details