JRC GMO-Amplicons | EURL GMFF

Record ID:	MN781001.1
Database:	nt	Go to original record
Description:	Eragrostis tef isolate T90-2 plastid, complete genome.
Taxon:	Eragrostis tef
Original size:	134350 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46367	46982	+	616 bp

Amplicon:

616 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagt ggttctcaaa
151   ctagaaccca aatgaaaagg ataggtgcag agactcaatg gaagctattc
201   taacgaatcg aggtgtaatt acgttgtgtt ggtaatgaaa ctccctctaa
251   atttgagaaa gaagggcttt atacatctaa tacacacgta tagatactga
301   catagcaaac gattaatcac agaacccata tcataatata ggttctttat
351   ttatttttta gaatgaaatt aggaatgatt atgaaataga aaattctgaa
401   ttttgattag aattattgtg aatccattcc actcgaatat tgagtaatca
451   aatccttcaa ttcattgttt ttgagatctt ttaaaaagag gattaatcgg
501   acgaggataa agagagagtc ccattctaca tgtcaatact gacaacaatg
551   aaatttctag taaaaggaaa atccgtcgac tttataagtc gtgagggttc
601   aagtccctct atcccc

View other 32 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	616	597	2.78e-08

Match details