JRC GMO-Amplicons | EURL GMFF

Record ID:	MN115425.1
Database:	nt	Go to original record
Description:	Pediomelum tenuiflorum voucher Jones 1026 chloroplast, partial genome.
Taxon:	Pediomelum tenuiflorum
Original size:	126144 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	13310	13894	-	585 bp

Amplicon:

585 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagtct tggtatggaa
51    acttaccaag tgagaacttt caaattcaga gaaaccctgg aattcacaac
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaga aaagttaaga
151   aagtgataat aaaaaaggga taggtgcaga gactcaatgg aagctattct
201   aacaaatgga gttgacgatt tttccttttt gcattaggaa aagaatcctt
251   ccataaaaat tccaggaatg gatcaaagag aaacatatat atactgaaat
301   actatttcaa ttgattaatg aagattcatt tgtgataaaa atattcacaa
351   atgcaaatga aagatgtgaa tcaaatcaat tccaagttga agaaaagatg
401   gaatattcat tgatcaaatt attcactcca tcataatctg atagatccct
451   tgaagaactg atcaatcaga cgagaataaa gatagagtcc tattctacat
501   gtcaatactg acaacaatga aatttatagt aagaggaaaa tccgtcgatt
551   taataaatcg tgagggttca agtccctcta tcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	585	566	2.63e-08

Match details