JRC GMO-Amplicons | EURL GMFF

Record ID:	MK609928.1
Database:	nt	Go to original record
Description:	Glechoma longituba chloroplast, complete genome.
Taxon:	Glechoma longituba
Original size:	153069 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47989	48542	+	554 bp

Amplicon:

554 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacca aggttcaaaa
151   aacgaaaaaa aaggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgcgccggt agaggaacct ttccatggaa attttagaaa
251   ggatgaagga taaacgcatc tattgaatac tatatcaaac aaatgattaa
301   tgttggccct aatctgtttt tttaatatga aaatgaaaaa atagatgtga
351   atttttttca cgttgaagaa aaaatagaat attcatcaac tcattcactc
401   catagtccgg tagatctttt aaagaactga ttaatcggac gagaataaag
451   atagagtccc attctacatg tcaataccga caacaatgaa atttatagta
501   agaggaaaat ccgtcgactt taaaaatcgt gagggttcaa gtccctctat
551   cccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	554	535	2.49e-08

Match details