JRC GMO-Amplicons | EURL GMFF

Record ID:	MK830556.1
Database:	nt	Go to original record
Description:	Dicliptera acuminata chloroplast, complete genome.
Taxon:	Dicliptera acuminata
Original size:	150738 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46238	46777	+	540 bp

Amplicon:

540 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tagtatggaa
51    acctactaag tgctaacttt caaattcaga gaaaccctgg aattaataaa
101   aaagggcaat cctgagccaa atcctctttt cgcaagataa aggttcagaa
151   aacgaaaaag ggataggtgc agagactcaa tggaagctgt tctaacttgg
201   ctgcattggt agaggaatcg ttccatcaaa acttccgaaa ggaaaaatct
251   atctattgaa tattatatca acagattaga tggcgcacat ctgtattttg
301   tatttgtatt tttttatatg caaaatggaa gaattgatgt gaattgattc
351   cacattgaag aacgagtcga atattcattt attcattcac caaacatcaa
401   atcattcact ccatagtcta gcggacgaga ataaagatag agtcccattc
451   cacatgtcaa taccggcaac aatgaaattt ttagtaagag gaaaatccgt
501   cgacttttaa aatcgtgagg gttcaagtcc ctctatcccc

View other 4 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.43e-08
primerB	100.0	20 bp	0	0	540	521	2.43e-08

Match details