JRC GMO-Amplicons | EURL GMFF

Record ID:	MK348612.1
Database:	nt	Go to original record
Description:	Sorghum bicolor isolate SbJ200 chloroplast, complete genome.
Taxon:	Sorghum bicolor
Original size:	140663 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49184	49706	+	523 bp

Amplicon:

523 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc cacttttttc aaaaaagtgg ttctcaaact
151   agaacccaaa ggaaaaggat aggtgcagag actcaatgga agctgttcta
201   acgaatcgaa gtaataacga ttaatcacag aacccatatt ataatatagg
251   ttctttattt tattttgaga atgaaattag gaatgattat gaaatagaaa
301   attcataatt tttttagaat tattgtgaat ctattccaat cgaatattga
351   gtaatcaaat ccttcaattc attgttttcg agatctttta aaaagtggat
401   taatcggacg aggataaaga gagagtccca ttctacatgt caatactgac
451   aacaatgaaa tttctagtaa aaggaaaatc cgtcgacttt ataagtcgtg
501   agggttcaag tccctctatc ccc

View other 9 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.35e-08
primerB	100.0	20 bp	0	0	523	504	2.35e-08

Match details