JRC GMO-Amplicons | EURL GMFF

Record ID:	MK348603.1
Database:	nt	Go to original record
Description:	Setaria italica isolate 56A chloroplast, complete genome.
Taxon:	Setaria italica
Original size:	139159 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48554	49166	+	613 bp

Amplicon:

613 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagt ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaattac gttgtgttgg tagtggaact ccctcgaaat
251   actagaaaga agggctttat acatttaata cacacgtata gatactaaca
301   tagcaaacga ttaatcacag aacccctatc ataatatagg ttctttattt
351   tagatttttt tttagaatga aattaggaat gattatgaaa tagaaaattc
401   tgaatttttt tagaattatt gtgaatccat tccaatcgaa tattgagtaa
451   tcaaatcctt caattcattg ttttgagatc aaaagtggat taatcgaacg
501   aggataaaga gagagtccca ttctacatgt caatactgac aacaatgaaa
551   tttctagtaa aaggaaaatc cgtcgacttt ataagtcgtg agggttcaag
601   tccctctatc ccc

View other 4 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	613	594	2.76e-08

Match details